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09395 Kinetic Analysis of Corrosive Biofilms by Bioluminescent Method of ATP Identification

Product Number: 51300-09395-SG
ISBN: 09395 2009 CP
Author: Vusal M. Shafiyev, Vagif M. Abbasov, Rufat E. Azizov and Elena N. Efremenko
Publication Date: 2009
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Recent research indicates that the quantification of Sulfate-Reducing Bacteria (SRB) and other corrosive bacteria in biofilms is of great practical and scientific relevance. Common microbial techniques based on selective cell cultivation are of limited usefulness for both quantification and characterization of environmental populations since the used methods are time-consuming and inconvenient for field trials or express analysis. To determine the total concentration of bacteria composing the corrosive biofilms, our research team developed a new approach was developed using bioluminescent method of ATP detection. The method also was optimised to determine microorganisms number in mixed cultures. The study of kinetics of corrosive biofilm formation in artificial pilot systems of field trials were carried out. The influence of the following factors on biofilm formation was revealed: microbial content of media, presence of oil, sulfide layer on the surface and metal type. The express screening of potential biocides active in relation to corrosive biofilms and the determination of minimal concentrations of tested compounds leading to destruction of corrosive biofilms were demonstrated herein.

Key words: corrosion, biofilms, bioluminescent method, SRB, ATP detection, kinetic analysis, minimal inhibitory concentrations, biocides
Recent research indicates that the quantification of Sulfate-Reducing Bacteria (SRB) and other corrosive bacteria in biofilms is of great practical and scientific relevance. Common microbial techniques based on selective cell cultivation are of limited usefulness for both quantification and characterization of environmental populations since the used methods are time-consuming and inconvenient for field trials or express analysis. To determine the total concentration of bacteria composing the corrosive biofilms, our research team developed a new approach was developed using bioluminescent method of ATP detection. The method also was optimised to determine microorganisms number in mixed cultures. The study of kinetics of corrosive biofilm formation in artificial pilot systems of field trials were carried out. The influence of the following factors on biofilm formation was revealed: microbial content of media, presence of oil, sulfide layer on the surface and metal type. The express screening of potential biocides active in relation to corrosive biofilms and the determination of minimal concentrations of tested compounds leading to destruction of corrosive biofilms were demonstrated herein.

Key words: corrosion, biofilms, bioluminescent method, SRB, ATP detection, kinetic analysis, minimal inhibitory concentrations, biocides
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