We describe the advancement of an activity-based quantitative polymerase chain reaction (qPCR) assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
Biocorrosion or microbiologically influenced corrosion (MIC) is a major problem in the oil and gas industry. Biofilms are the culprits of MIC. In this work, D-amino acids were used to enhance two biocides, alkyldimethylbenzylammonium chloride (ADBAC) and tributyl tetradecyl phosphonium chloride (TTPC), to treat a field biofilm consortium on C1018 carbon steel coupons.