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Comparing in-situ Methods for Monitoring Effectiveness of Biocide Treatment

In this paper we describe a case study in which we compared several available methods including viabilitypolymerase chain reactionfor measuring the effectiveness of biocide treatment.

Product Number: 51317--9114-SG
ISBN: 9114 2017 CP
Author: Sabine Doddema
Publication Date: 2017
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Microbial Influenced Corrosion (MIC) is difficult to control. Therefore biocides are often added as a preventive measure to exterminate all microbial activity and with that also MIC. The effectiveness of the biocide treatment is often measured by culturing methods however one of the disadvantages of these methods is that the biocide is not separated from the test sample therefore influencing the biocide effectiveness test since the biocide has an extended contact time in these tests. DNA technology such as QPCR is a good alternative however this gives an over-estimation of the surviving microorganisms since not all DNA from dead microorganisms will be destroyed. Another approach is the measurement of RNA to screen for the effectiveness of biocide treatment however RNA is unstable which makes the interpretation difficult. Here we compare traditional methods such as culturing and cATP methods with DNA and RNA based methods including viability PCR (vPCR). VPCR is a relatively new method that can distinguish viable cells from dead cells by using nucleotide based (DNA or RNA) technology in combination with a dye. In this paper we describe a case study in which we compared several available methods including vPCR for measuring the effectiveness of biocide treatment.

Microbial Influenced Corrosion (MIC), well injection, produced water, biocide, monitoring

Microbial Influenced Corrosion (MIC) is difficult to control. Therefore biocides are often added as a preventive measure to exterminate all microbial activity and with that also MIC. The effectiveness of the biocide treatment is often measured by culturing methods however one of the disadvantages of these methods is that the biocide is not separated from the test sample therefore influencing the biocide effectiveness test since the biocide has an extended contact time in these tests. DNA technology such as QPCR is a good alternative however this gives an over-estimation of the surviving microorganisms since not all DNA from dead microorganisms will be destroyed. Another approach is the measurement of RNA to screen for the effectiveness of biocide treatment however RNA is unstable which makes the interpretation difficult. Here we compare traditional methods such as culturing and cATP methods with DNA and RNA based methods including viability PCR (vPCR). VPCR is a relatively new method that can distinguish viable cells from dead cells by using nucleotide based (DNA or RNA) technology in combination with a dye. In this paper we describe a case study in which we compared several available methods including vPCR for measuring the effectiveness of biocide treatment.

Microbial Influenced Corrosion (MIC), well injection, produced water, biocide, monitoring

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