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51317--9802-Determining Biocide Selection and Dosage Recommendation via Planktonic and Sessile Kill Studies and Subsequent Biofilm Regrowth: A Case Study

A three-phase production system in the North Sea was experiencing multiple leaks in the topside separation facilities. To control the microbial contamination in the system, an initial planktonic kill study was performed to select the best possible biocides to provide immediate microbial mitigation to the field.

 

Product Number: 51317--9802-SG
ISBN: 9802 2017 CP
Author: Zach Broussard
Publication Date: 2017
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A three phase production system in the North Sea was experiencing multiple leaks in the topside separation facilities. A root cause analysis using a combination of ATP photometry and DNA-based techniques was performed and the underlying source responsible for the failures was determined to be microbially influenced corrosion. To control the microbial contamination in the system an initial planktonic kill study was performed to select for the best possible biocides to provide immediate microbial mitigation to the field. Upon implementation resulting field data indicated effective initial control. Treatment efficiency was further corroborated by follow up laboratory sessile studies to confirm the biocide selection and to determine the frequency of biocide injection needed to maintain control over the sessile population. For these experiments representative biofilms were cultivated in two separate CDC biofilm reactors and used to evaluate biocide candidates based on sessile bacterial kill biofilm removal and to monitor the regrowth of the biofilm following the biocide injection. The data obtained from monitoring the biofilm regrowth was utilized to recommend a biocide injection frequency.

Key words: Biofilms, Sessile Kill Study, CDC Biofilm Reactor, CLSM, ATP Quantification, MIC, Biofilm Regrowth, Injection Frequency, Biocide

A three phase production system in the North Sea was experiencing multiple leaks in the topside separation facilities. A root cause analysis using a combination of ATP photometry and DNA-based techniques was performed and the underlying source responsible for the failures was determined to be microbially influenced corrosion. To control the microbial contamination in the system an initial planktonic kill study was performed to select for the best possible biocides to provide immediate microbial mitigation to the field. Upon implementation resulting field data indicated effective initial control. Treatment efficiency was further corroborated by follow up laboratory sessile studies to confirm the biocide selection and to determine the frequency of biocide injection needed to maintain control over the sessile population. For these experiments representative biofilms were cultivated in two separate CDC biofilm reactors and used to evaluate biocide candidates based on sessile bacterial kill biofilm removal and to monitor the regrowth of the biofilm following the biocide injection. The data obtained from monitoring the biofilm regrowth was utilized to recommend a biocide injection frequency.

Key words: Biofilms, Sessile Kill Study, CDC Biofilm Reactor, CLSM, ATP Quantification, MIC, Biofilm Regrowth, Injection Frequency, Biocide

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