We describe the advancement of an activity-based quantitative polymerase chain reaction (qPCR) assay which can distinguish live from dead corrosion influencing microorganisms in oil and gas pipeline environments. We discuss the limitations and possible future optimization methods for Propidium monazide-qPCR techniques in the industry.
Fusion bonded epoxy (FBE) coatings protect the underlying metal from corrosion. The lack of research on the microbial impact of pipeline coating failures leaves a significant knowledge gap. We analyzed two FBE coating samples from buried steel transmission pipelines with unusually rapid external pitting.